The present article presents a study aiming to inquire the intra-business training as a means to implement Job Rotation (JR), in the Public Sector and specifically the ΟΑΕΕ organization. The focus to the public sector is given because it is the unique provider of some services (ie justice, security, social care etc.), and at the same time it is in a process of change inside the new European framework that is being created. The sample of the study is made of ΟΑΕΕ employees, 138 persons, of which 102 participated at least once in a JR program, while 36 never did so. From the findings, it was concluded that intra-business training provides to the employees knowledge and skills, which contribute not only to the improvement of provided services, but also to the modernization and updating of the organization. In this framework Job rotation provides to the trainee employees knowledge, skills and experience, while it is an important motivational factor. The study is a first step towards investigating the effective use of training tools in the Greek Public Sector from the point of Greek Bibliography in order to help improve training and skills of its human resources.