The purpose of this paper is to identify the factors that determine entrepreneurial intention and examine the effects of age and prior working experience on the formation of entrepreneurial intention. A questionnaire-based survey was employed for the data collection. A total of 171 university students from a Business School in Greece participated in the survey. The findings of our research showed that perceived behavioral control and attitude are significantly influencing entrepreneurial intention. Additionally, our analysis indicates that age and prior working experience affect entrepreneurial intention. The contribution of this study concerns the illumination of the scarcely addressed in the literature relationship between age and work experience with entrepreneurial intention.