The purpose of this study is to discern the similarities and differences in sources of motivation between employees in construction sites versus office employees using the Herzberg Two-Factor Theory. The study further analyses the effects of motivation factors, considering variables such as job position, gender and nationality. The present research analyses the responses of 326 office and construction site employees of various specialties. The results indicate that workplace physical environment plays an important role in employees’ motivation. Office employees and construction site employees are affected differently by the items in Herzberg’s two-factor theory. The findings of the study are expected to help managers make better use of their incentive repertoire, in accordance with the specific group needs, whether they are office employees or field workers. Research limitations include the need for a larger sample size since the present study does not allow for generalizable conclusions, partly due to the difficulty in conducting survey studies in the highly complex construction work environment.