The purpose of this study is to examine the impact of Entrepreneurship Education (EE) on the intention to start a business among university students of the Tourism Department. We used the theory of Planned Behavior (TPB), in measuring each of the theory’s components before the intervention and after. A sample of 77 students answered the questionnaire both at the beginning of the semester and at the end of it. The results suggest that the intervention had a positive impact on Personal Attitude (PA), on Perceived Behavioral Control (PBC) and on Entrepreneurial Intention (EI). No significant change was observed in Social Norms (SN). The findings have implications both for policy makers and academics. The former can help foster entrepreneurship and through that economic growth, making provisions for the availability of EE programs both inside and outside the university ecosystems. The latter can devote more resources in research and teaching EE programs, experimenting with teaching methods and new technologies, so as to increase the student awareness of the entrepreneurial career option. The contribution of this study lies in that no other study has used a longitudinal method in measuring the effect of EE on EI and its antecedents among tourism management students.