Behavioral scholars propose that entrepreneurial intention (EI) is the best way to predict an entrepreneurial action. However, it has been observed that students at the beginning of their studies show increased entrepreneurial intention, especially when attending an entrepreneurship course, while after its completion and before completing their studies, their intention is changed. The present research attempts to extend the knowledge of how Entrepreneurial Intention is changing over time. A qualitative research was conducted with the use of structured interviews with 140 university students who had attended the entrepreneurship and innovation course two years before, to evaluate the changes and the reasons for changing their entrepreneurial intention during the last two years. Data were analyzed with the use of NVivo coding software. The findings suggest that factors such as the attractiveness of the entrepreneurial idea, the cohesion of the teams, the teaching methods used, and the support that students receive from their university as well as some personality characteristics were significant factors that caused changes in students’ entrepreneurial intention within the last two years of their studies. The main contribution of the study is the examination of the changes in students’ entrepreneurial intention and the highlighting of the challenges that appear at different times of their studies.